Table 3. Kinetic Parameters Obtained from the Modeling of the Conjugation of A (Dextm) to B (ADH, PDHA or A5M-ADH or A5M-PDHA) Using Different Equivalents of Ba.
| A + B ↔ E |
A + B ↔ Z |
E ↔ Pyr |
Z ↔ Pyr |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| A | B (2×) | k1 (h–1) | k–1 (h–1) | k2 (h–1) | k–2 (h–1) | k3 (h–1) | k–3 (h–1) | k4 (h–1) | k–4 (h–1) | equilibrium yield (%) |
| Dext5 | ADH | 1.5 × 10–3 | 1.0 × 101 | 1.5 × 10–4 | 1.0 × 101 | 1.1 × 102 | 1.1 × 10° | 1.1 × 102 | 1.1 × 10° | 35 |
| Dext5 | PDHA | 2.9 × 10–3 | 2.0 × 10–3 | 4.0 × 10–4 | 1.5 × 10–1 | 2.0 × 10° | 6.8 × 10° | 2.7 × 101 | 2.0 × 101 | 87 |
| A | B (1×) | |||||||||
| Dext6 | A5M-ADH | 3.0 × 10–3 | 1.7 × 101 | 3.0 × 10–4 | 1.7 × 101 | 1.1 × 102 | 1.1 × 10° | 1.1 × 102 | 1.1 × 10° | 15 |
| Dext6 | A5M-PDHA | 1.1 × 10–2 | 5.0 × 10–2 | 1.5 × 10–3 | 1.0 × 10–1 | 1.9 × 10° | 4.5 × 10° | 2.7 × 101 | 1.5 × 101 | 66 |
a
Reactions were performed at pH 4.0, RT. E: E-hydrazone/oxime. Z: Z-hydrazone/oxime. Pyr: N-pyranoside.