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Shake flask experiments of A. simplex: growth on GYA or GEM III N media, at pH 6.0 or 7.0, at 26 °C (A) or at 30 °C (B) and the correspondent bioconversion percentages of 0.1 g·L−1 HC to prednisolone (C). HC was added at time zero (* indicates p < 0.05; ** indicates p < 0.01).
