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. 2020 Oct 24;21(21):7896. doi: 10.3390/ijms21217896

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Measurements of pH value and bioluminescence of luciferin analogues with AkaLuc. pH values of 10 mM solutions of D-luciferin, TokeOni and seMpai (n = 3) (A). In vitro reaction of luciferin analogues with AkaLuc (B). The table shows the value of Michaelis constant (K_m) and relative maximal velocity (rel. V_max) for luciferin analogues with AkaLuc.