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Chromatographic separation of glucosylsphingosine (lyso-Gb1; 5 μg on column, retention time: 23.78 min) and psychosine (5 μg on column, retention time: 24.36 min) commercial standards analyzed by time-of-flight mass spectrometry (MS-TOF) with a mass window of 0.05 Da. Peaks associated with each compound were confirmed by individual analysis of each standard. ES+ = positive electrospray.
