Table 3.
Mass spectrometry (MS) parameters for the quantitation of compounds present in the plasma samples analyzed during the metabolomic study.
| Parameters | Description |
|---|---|
| Scan Mode | MS-TOF |
| Ionization Mode | Electrospray Ionization |
| Polarity | Positive |
| Analyzer Mode | V |
| Dynamic Range | Extended |
| Capillary Voltage | 1.4 kV |
| Sampling Cone Voltage | 10 V |
| Extraction Cone Voltage | 5.0 V |
| Source Temperature | 150 °C |
| Desolvation Temperature | 450 °C |
| Cone Gas Flow | 30 L/h |
| Desolvation Gas Flow | 700 L/h |
| Trap Collision Energy | 4.0 V |
| Transfer Collision Energy | 2.0 V |
| Data Format | Centroid |
| Mass Range | 50–1000 Da |
| Scan Time | 0.1 s |
| Lock mass | |
| Compound | Terfenadine (500 nM) |
| Exact Mass | 472.3215 Da |
| Solvent | 94.5:5:0.5 H2O:ACN:FA |
| Scan Time | 0.5 s |
| Interval | 5.0 s |
| Sampling Cone Voltage | 5 V |
| Trap Collision Energy | 10 |
| Mass Window | ±0.2 Da |
| Scan Average | 3 |