Figure 4.
Isothermal titration calorimetry (ITC) demonstrated binding of 7, 9, and 10 to the recombinant HNF4α LBD protein, confirming their HNF4α-mediated activity. Recombinant HNF4α LBD protein (10–20 µM) was titrated with ligands (50–200 µM).
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