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. 2020 Oct 23;21(21):7885. doi: 10.3390/ijms21217885

Figure 4.

Figure 4

MSC-derived EV analysis. (A) The size of events was obtained by transforming .fcs files through the Rosetta Calibration system. The size of EVs was represented as a histogram and a gate covering EV dimensions was drawn (100–1000 nm). (B) Events displaying a diameter in the range 100–1000 nm were then analyzed on a Phalloidin FITC-H/LCD-H dot-plot and LCD+/Phalloidin- events were identified as EVs. (C) EVs were analyzed for their positivity to CD90 expression: (D) the dot-plot represents the FMO for CD90. The figure is representative of at least three separate experiments.