Figure 3.
Bio‐functional testing corroborates high‐HMGB1 (high mobility group box 1) and low‐LAMC1 (laminin gamma chain 1) expression in abnormal subjects (A) vs normal subjects (N). Left, HMGB1 content of culture medium over blood outgrowth endothelial cells measured ± lipopolysaccharide stimulation for 24 hours. Baseline release (white bars) tended to be higher for abnormal vs normal subjects (P=0.158). In response to lipopolysaccharide (black bars), abnormal blood outgrowth endothelial cells released 13.3‐fold greater HMGB1 (P=8.5×10−5). The specific subjects studied are indicated in red in the embedded HMGB1 expression plot. Right, LAMC1 deposition into collagen subendothelial matrix by abnormal blood outgrowth endothelial cells over 14 days incubation was, on average, one tenth that by normal blood outgrowth endothelial cells. LPS indicates lipopolysaccharide.