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. 2020 Aug 4;9(16):e016419. doi: 10.1161/JAHA.120.016419

Figure 6. NULP1 (nuclear localized protein 1) interacts with and inactivates nuclear factor of activated T cells 3 (NFAT3) during cardiac hypertrophy.

Figure 6

A, Representative western blots for coimmunoprecipitation (co‐IP) assays screening the binding of NULP1 with different NFATs by incubating HA‐NULP1 and the indicated NFAT plasmids in vitro. B, NFAT‐luciferase (NFAT‐luc) activity in H9C2 cells transfected with NFAT1 alone or in combination with NULP1. C, NFAT‐luciferase activity in H9C2 cells transfected with NFAT3 alone or in combination with NULP1. D and E, Schematic of full‐length and truncated NULP1 (D, left) and NFAT3 (E, left) and representative western blots for mapping analyses showing the binding domains of NULP1 to NFAT3 (D and E, right). F, NFAT‐luciferase activity in H9C2 cells transfected with NFAT3 alone or in combination with full‐length and truncated NULP1. For all statistical plots, the data are presented as the mean±SD; in (B, C, and F) **P<0.01 and n.s. indicate no significance; the statistical analysis was performed using a 1‐way ANOVA.