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. 2020 Aug 4;9(16):e016419. doi: 10.1161/JAHA.120.016419

Figure 8. The regulatory effect of NULP1 (nuclear localized protein 1) on cardiac hypertrophy is dependent on nuclear factor of activated T cells 3 (NFAT3).

Figure 8

A, Left, representative images of immunofluorescence staining for α‐actinin (green) and DAPI (blue) in cultured neonatal rat ventricular myocytes (NRVMs) infected with adenovirus AdshNulp1 alone or in combination with AdshNfat3 in response to PBS (phosphate buffer saline) Ang II (angiotensin II, 1 μmol/L) stimuli for 48 hours. Scale bar, 20 μm. Right, relative cell size of cultured NRVMs in the indicated groups. n>50 cells per group. B, quantitative polymerase chain reaction (qPCR) analyses of the mRNA levels of atrial natriuretic peptide (Anp) and Myh7 in the indicated groups. n=3 samples per group. C, Left, representative images of immunofluorescence staining for α‐actinin (green) and DAPI (blue) in cultured NRVMs infected with AdNulp1 alone or in combination with AdNfat3 followed by 48 hours of PBS or Ang II treatment. Scale bar, 20 μm. Right, relative cell size of cultured NRVMs in the indicated groups. n>50 cells per group. D, qPCR analyses of the mRNA levels of Anp and Myh7 in the indicated groups. n=3 samples per group. For all statistical plots, the data are presented as the mean±SD; in (A through D) **P<0.01; the statistical analysis was performed using a 1‐way ANOVA. In (B and D) the dotted line indicates the mRNA levels in AdshRNA PBS or AdVector PBS group, which were normalized to 1.