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. 2020 Aug 12;9(16):e015841. doi: 10.1161/JAHA.119.015841

Figure 6. Laminin‐conjugated engineered cardiac tissue (L‐ECT) induced angiogenesis and angiogenic cytokine expression in myocardial infarction (MI) model.

Figure 6

A, Left: histological sections of 3‐dimensional engineered cardiac tissues after being transplanted on the rat heart with MI. Right: immunostaining of human nuclei (red), TnT or Trop T (troponin T; green), and nuclei (blue). Bar=50 µm. B, Immunostaining of isolectin B4 (ILB4; red), TnT (green), and nuclei (blue). Bar=50 µm. C, Capillary formation at the peri‐infarct zone in MI heart 4 weeks after transplantation. Sections are immunostained with von Willebrand factor. Bar=50 mm. D, Quantitative polymerase chain reaction analysis of angiogenic cytokine‐related gene expression in peri‐infarct zone: VEGF (vascular endothelial growth factor), PDGF (placental growth factor), FGF (fibroblast growth factor), and SDF‐1 (stromal cell–derived factor 1) (L‐ECT group, n=7; ECT group, n=7; control group, n=7). DAPI indicates 4',6‐diamidino‐2‐phenylindole; and HuNu, human nuclear.