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. 2020 Nov 2;16(11):e1008214. doi: 10.1371/journal.pcbi.1008214

Fig 3. The functional workflow links tools in Galaxy used for functional prediction.

Fig 3

Inputs for the functional workflow are gene calls paired with their genome. These are piped through five sub-paths within the analysis. 1) The BLASTn path uses full genomic nucleotide sequence (light blue noodles). 2) The BLASTp protein analysis against curated (UniProtKB SwissProt) and sequence-inclusive databases (NCBI nonredundant (nr)) (dark blue noodles). 3) The search for interrupted genes like introns compiles separate CDS hits to the same protein (yellow noodles). 4) A directed search for spanin proteins using TMHMM, lipobox-finding (using LipoP and a motif search), and BLASTp against a curated database (magenta and pink noodles). 5) Domain analysis plots comprehensive TMHMM outputs and InterProScan results for conserved domains and signatures (green and purple noodles, respectively).