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. 2020 Nov 2;16(11):e1009194. doi: 10.1371/journal.pgen.1009194

Fig 3. Alternative splicing pattern of Bmdsx and qRT-PCR analysis of BmMasc and putative target genes of Bmdsx.

Fig 3

(A) Relative expression of BmMasc in gonads of WT and ΔBmGtsf1 at wandering stage. (B) Splicing patterns of Bmdsx in gonads of WT and BmGtsf1 mutants at wandering stage detected by RT-PCR. (C-H) Relative expression of BmPBP1 (C), BmOR1(D), BmOR3 (E), BmOR19 (F), BmOR30 (G) and BmVg (H) in BmGtsf1 female mutants. RNA extracted from antenna of adults was used for qRT-PCR analyses in Fig 3C–Fig 3G, while the expression of BmVG was detected in fat body at wandering stage. Error bars represent ±SD. The asterisks represent statistically significant differences with p < 0.001.