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. 2020 Nov 2;16(11):e1009194. doi: 10.1371/journal.pgen.1009194

Fig 4. BmGtsf1 regulate female sex determination via Fem piRNA.

Fig 4

(A-C) Relative mRNA levels of BmPSI (A), BmIMP (B) and BmImpM (C, male-specific BmIMP) in gonads of WT and mutants at wandering stage. (D) RT-PCR analysis of BmImpM in gonads of WT and mutants at wandering stage. (E and F) Relative abundance of Fem piRNA (E) and Masc piRNA (F) in ovary and fat body of female mutant at wandering stage, the small RNA U6 was used as the internal reference. (G) Splicing patterns of Bmdsx and expression of Fem precursor in WT and BmGtsf1 mutant embryoes at 24hpo detected by RT-PCR. The asterisks represent statistically significant differences with p < 0.001. Error bars represent ±SD.