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. 2020 Aug 4;166(10):995–1003. doi: 10.1099/mic.0.000956

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© 2020 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 5. — T4P are not absolutely required for natural transformation. Static (a–c) or shaking (a) broth cultures were incubated at 37 °C for 24 h with 10 µg pUCPSK and the number of (a, b) carbenicillin-resistant transformants or (c) aggregates determined. For (a, b) the mean of each set of technical triplicates was calculated to give an n≥3, which is presented as mean±sem. For (a) P >0.05 (ns); Mann–Whitney U-test. For (b) ** P <0.005; Mann-Whitney U-test compared to PAK. For (c) the total number of aggregates from 60 random fields imaged on 3 separate days (20 fields/day) is presented.