KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Flag M2-HRP | Sigma #A8592 | AB_439702 |
| Drosophila IntS1 | Ezzeddine et al., 2011 | |
| Drosophila IntS4 | This paper | |
| Drosophila IntS7 | This paper | |
| Drosophila IntS8 | This paper | |
| Drosophila IntS9 | Ezzeddine et al., 2011 | |
| Drosophila IntS11 | Ezzeddine et al., 2011 | |
| Drosophila IntS12 | Chen et al., 2012 | |
| Drosophila PR65 | This paper | |
| PP2Ac | Cell Signaling #2038 | AB_2169495 |
| alpha-tubulin | abcam #ab15246 | AB_301787 |
| GAPDH (GA1R) | ThermoFisher #MA5-15738 | AB_10977387 |
| RNA Pol II CTD Tyr1-p (3D12) | Active motif #61383 | AB_2793613 |
| RNA Pol II CTD Ser2-p (3E10) | Active motif #61083 | AB_2687450 |
| RNA Pol II CTD Thr4-p (6D7) | Active motif #61361 | AB_2750848 |
| RNA Pol II CTD Ser5-p (3E8) | Active motif #61085 | AB_2687451 |
| RNA Pol II CTD Ser7-p (4E12) | Active motif #61087 | AB_2687452 |
| RNA Pol II CTD Ser7-p (3D4A12) | Active motif #61703 | AB_2793742 |
| Spt5-Ser707P | This paper | |
| Spt5-Thr806P | This paper | |
| Spt5 | BD #611107 | AB_398420 |
| hINTS1 | Bethyl #A300–361A | AB_2127258 |
| hINTS3 | Proteintech #16620–1 | AB_2127274 |
| hINTS4 | Bethyl #A301–296A | AB_937909 |
| hINTS7 | Bethyl #A300–271A | AB_2127399 |
| hINTS10 | Proteintech #15271–1 | AB_2127260 |
| hINTS11 | Bethyl #A301–274 | AB_937779 |
| hPR65 (81G5) | Cell Signaling #2041 | AB_2168121 |
| siRNA | ||
| Universal Negative Control #2 | Sigma | Cat. #SIC002 |
| Human IntS8 (NM_017864) | Sigma | SASI_Hs01_00121954 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| AzNTPs | TriLink Technologies | Cat. #K-1005 |
| Biotin-11-NTPs | Perkin Elmer | Cat. #NEL54(2/3/4/5)001 |
| Okadaic Acid | Cell Signaling | Cat. #5934S |
| Calyculin A | Cell Signaling | Cat. #9902S |
| Phendione | Sigma | Cat. #496383 |
| Critical Commercial Assays | ||
| NEB Next Ultra II DNA library kit | NEB | Cat. #E7103S |
| PP2A Immunoprecipitation Phosphatase Assay Kit | Sigma | Cat. #17–313 |
| Deposited Data | ||
| Raw and analyzed data | This paper | GEO: GSE150844 |
| Raw image files | Mendeley | http://dx.doi.org/10.17632/bt76mktdfx.1 |
| RNA-seq from IntS9 and IntS11-depleted DL1 cells, IntS1 and IntS12 ChIP-seq | Elrod et al., 2019 | GEO: GSE114467 |
| Experimental Models: Cell Lines | ||
| DL1 | Dr. Sara Cherry, UPenn | |
| DL1 FLAG-eGFP | This paper | |
| DL1 FLAG-IntS8-WT | This paper | |
| DL1 FLAG-IntS8-WFEF/A | This paper | |
| S2-DGRC clone 6 | DGRC | |
| S2 Flag-PR65 | This paper | |
| S2 Flag-IntS1 | This paper | |
| S2 Flag-IntS5 | This paper | |
| S2 Flag-IntS8 | This paper | |
| S2 Flag-IntS8-WFEF/A | This paper | |
| S2 Flag-IntS11 | This paper | |
| 293T | ATCC | CRL-3216 |
| 293T CRISPR-en-Flag-PR65 | This paper | |
| 293T CRISPR-en-Flag-INTS1 | This paper | |
| 293T CRISPR-en-Flag-INTS5 | This paper | |
| 293T CRISPR-en-Flag-INTS8 | This paper | |
| 293T CRISPR-en-Flag-INTS11 | This paper | |
| 293T Flag-INTS8-WT | This paper | |
| 293T Flag-INTS8-WFEF/A | This paper | |
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| Table_S1 | This paper | |
| Recombinant DNA | ||
| pUB-3xFLAG vector | Chen et al., 2012 | |
| pUB-3xFLAG-dIntS8-WT | This paper | |
| pUB-3xFLAG-dIntS8-WFEF/A | This paper | |
| pMT-3xFLAG-puro vector | Elrod et al., 2019 | |
| pMT-3xFLAG-dIntS8-WT-RESC-puro | This paper | |
| pMT-3xFLAG-dIntS8-WFEF/A-RESC-puro | This paper | |
| pMT-3xFLAG-eGFP-puro | This paper | |
| pcDNA6–3xFlag-hINTS8-WT | Oegema et al., 2017 | |
| pcDNA6–3xFlag-hINTS8-WFEF/A | This paper | |
| Software and Algorithms | ||
| bowtie 1.2.2 | Langmead et al., 2009 | |
| R v3.3.1 | www.r-project.org | |
| Rstudio v1.0.136 | www.rstudio.com | |
| featureCounts | Liao et al., 2014 | |
| DESeq2 | Love et al., 2014 | |
| MISO | Katz et al., 2010 | |
| Prism v8.1.2 | GraphPad | |
| Partek Genomics Suite v6.15.0127 | www.partek.com | |