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. 2020 Oct 26;9:e59333. doi: 10.7554/eLife.59333

Key resources table.

Reagent type
(species) or resource
Designation Source or reference Identifiers Additional information
Antibody Anti-phospho-p42/44 (Rabbit polyclonal) Cell Signaling Cat#: 9101, RRID:AB_331646 WB (1:2500)
Antibody Anti-HA (Mouse monoclonal) Roche Cat# 11666606001, RRID:AB_514506 WB (1:5000)
Antibody HRP anti-mouse (Goat polyclonal) Bio-Rad Cat#: 170–6516, RRID:AB_11125547 WB (1:10000)
Antibody HRP anti-rabbit (Goat polyclonal) Bio-Rad Cat#: 170–5046, RRID:AB_11125757 WB (1:15000)
Chemical compound, drug Blankophor Bayer Blankophor BA 267%
Chemical compound, drug KCl Merck Cat#: 104936
Chemical compound, drug Caffeine Sigma-Aldrich Cat#: W222402
Chemical compound, drug Caspofungin Sigma-Aldrich Cat#: SML0425
Chemical compound, drug Latrunculin A Sigma-Aldrich Cat#: L5163
Chemical compound, drug Hydroxyurea Sigma-Aldrich Cat#: H8627
Chemical compound, drug Soybean lectin Sigma Aldrich Cat#: L2650
Commercial assay, kit Ni-NTA Novagen Cat#: 70666
Software, algorithm GraphPad Prism GraphPad Prism (https://graphpad.com) RRID:SCR_015807
Software, algorithm ImageJ ImageJ (http://imagej.nih.gov/ij/) RRID:SCR_003070
Other μ-Slide eight well Ibidi Cat#: 80826