Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Antibody | Anti-phospho-p42/44 (Rabbit polyclonal) | Cell Signaling | Cat#: 9101, RRID:AB_331646 | WB (1:2500) |
Antibody | Anti-HA (Mouse monoclonal) | Roche | Cat# 11666606001, RRID:AB_514506 | WB (1:5000) |
Antibody | HRP anti-mouse (Goat polyclonal) | Bio-Rad | Cat#: 170–6516, RRID:AB_11125547 | WB (1:10000) |
Antibody | HRP anti-rabbit (Goat polyclonal) | Bio-Rad | Cat#: 170–5046, RRID:AB_11125757 | WB (1:15000) |
Chemical compound, drug | Blankophor | Bayer | Blankophor BA 267% | |
Chemical compound, drug | KCl | Merck | Cat#: 104936 | |
Chemical compound, drug | Caffeine | Sigma-Aldrich | Cat#: W222402 | |
Chemical compound, drug | Caspofungin | Sigma-Aldrich | Cat#: SML0425 | |
Chemical compound, drug | Latrunculin A | Sigma-Aldrich | Cat#: L5163 | |
Chemical compound, drug | Hydroxyurea | Sigma-Aldrich | Cat#: H8627 | |
Chemical compound, drug | Soybean lectin | Sigma Aldrich | Cat#: L2650 | |
Commercial assay, kit | Ni-NTA | Novagen | Cat#: 70666 | |
Software, algorithm | GraphPad Prism | GraphPad Prism (https://graphpad.com) | RRID:SCR_015807 | |
Software, algorithm | ImageJ | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
Other | μ-Slide eight well | Ibidi | Cat#: 80826 |