Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Dot1l | GenBank | ID: 208266 | |
| Strain, strain background (Escherichia coli) | BL21(DE3) | NEB | C2527H | |
| Strain, strain background (Mus musculus) | ES cell line Dot1ltm1a(KOMP) | KOMP Repository | MMRRC:054749-UCD | |
| Genetic reagent (Mus musculus) | Dot1l-G55L | This lab | ||
| Cell line (Homo sapien) | HEK293 | UCB Cell Culture Facility | RRID:CVCL_0045 | Authenticated using STR analysis |
| Cell line (Mus musculus) | 3T3-L1 | UCB Cell Culture Facility | RRID:CVCL_0123 | Authenticated using STR analysis |
| Cell line (Mus musculus) | BAT cells | Shingo Kajimura Ohno et al., 2013 |
Harvard; authenticated using methods described in Aune et al., 2013 | |
| Transfected construct (Mus musculus) | Ad-m-Dot1l-shRNA | Vector Biolabs | shADV-257398 | |
| Transfected construct (Mus musculus) | Ad-Zc3h10-flag | Vector Biolabs | ADV-276549 | |
| Transfected construct (Mus musculus) | Ad-m-Dot1l | Vector Biolabs | ADV-257398 | |
| Antibody | Anti-Dot1l (rabbit monoclonal) | Cell signaling | Cat# 77087 RRID:AB_2799889 |
1:1000 |
| Antibody | Anti-GAPDH (rabbit monoclonal) | Cell signaling | Cat# 5174S RRID:AB_10622025 |
1:1000 |
| Antibody | Anti-UCP1 (rabbit polyclonal) | Abcam | Cat# 10983 RRID:AB_2241462 |
1:1000 |
| Antibody | Anti-H3K79me2 (rabbit polyclonal) | Abcam | Cat# ab3594 RRID:AB_303937 |
ChIP: 5 µg (5 µl) |
| Antibody | Anti-H3K79me3 (rabbit monoclonal) | Abcam | Cat# ab2621 RRID:AB_303215 |
ChIP: 5 µg (5 µl) |
| Antibody | Anti-Zc3h10 (rabbit polyclonal) | Thermo Fishcer | Cat# PA5-31814 RRID:AB_2549287 |
1:1000 |
| Recombinant DNA reagent | HA-Dot1l expression vector | Genecopoeia | EX-Mm14067-M07 | |
| Sequence-based reagent | Dot1l_F | This paper | RT-qPCR primer | CGA CTA ATG CTG CAC CTC CT |
| Sequence-based reagent | Dot1l_R | This paper | RT-qPCR primer | AGG AGT AGT GGT GTG GCT CA |
| Sequence-based reagent | Ucp1_F | This paper | RT-qPCR primer | ACT GCC ACA CCT CCA GTC ATT |
| Sequence-based reagent | Ucp1_R | This paper | RT-qPCR primer | CTT TGC CTC ACT CAG GAT TGG |
| Sequence-based reagent | Tfam_F | This paper | RT-qPCR primer | GTC CAT AGG CAC CGT ATT GC |
| Sequence-based reagent | Tfam_R | This paper | RT-qPCR primer | CCC ATG CTG GAA AAA CAC TT |
| Sequence-based reagent | Nrf1-F | This paper | RT-qPCR primer | GAC AAG ATC ATC AAC CTG CCT GTA G |
| Sequence-based reagent | Nrf1-R | This paper | RT-qPCR primer | GCT CAC TTC CTC CGG TCC TTT G |
| Sequence-based reagent | Zc3h10-F | This paper | RT-qPCR primer | CGA CTA ATG CTG CAC CTC CT |
| Sequence-based reagent | Zc3h10-R | This paper | RT-qPCR primer | AGG AGT AGT GGT GTG GCT CA |
| Commercial assay or kit | NE-PER Nuclear and Cytoplasmic Extraction Kit | Thermo Fisher | 78833 | |
| Commercial assay or kit | SimpleChIP Plus Sonication Chromatin IP Kit | Cell signaling | 56383S | |
| Commercial assay or kit | Dual-Luciferase Kit | Promega | E1980 | |
| Commercial assay or kit | Nextera DNA library Preparation Kit | Illumina | 15028212 | |
| Chemical compound, drug | EPZ5676 | MedChemExpress | HY-15593 | |
| Software, algorithm | Partek Flow Genomics Suite | Partek | RRID:SCR_011860 | |
| Other | Rectal probe | Physitemp | BAT-12 | For mice |