Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Rattus norvegicus, male) | Fischer 344 CDF | NIA Aged Rodent Colony | ||
| Software, algorithm | Fusion 4.0 | Accuscan Instruments, Inc | ||
| Software, algorithm | Rota-Rod Treadmill Control Software | MED-Associates, Inc | SOF-ENV-575 | |
| Commercial assay or kit | QIAamp DNA Blood Mini Kit | Qiagen | 51106 | |
| Commercial assay or kit | Genomic DNA Clean & Concentrator (gDCC) | Zymo | D4010 | |
| Commercial assay or kit | Qubit 1x dsDNA HS Assay Kit | Thermo Fisher Scientific |
Q33231 |
|
| Antibody | Anti-rat CD3-FITC (Mouse monoclonal) | BioLegend | Cat#: 201403 | FACS (1.0 ul/test) |
| Antibody | Anti-rat CD25-PE (Mouse monoclonal) | BioLegend | Cat#: 202105 | FACS (1.25 ul/test) |
| Antibody | Anti-rat CD8a-PerCP (Mouse monoclonal) | BioLegend | Cat#: 201712 | FACS (5.0 ul/test) |
| Antibody | Anti-rat CD11b/c-PE-Cy7 (Mouse monoclonal) | BioLegend | Cat#: 201818 | FACS (1.25 ul/test) |
| Antibody | Anti-rat CD4-APC-Cy7 (Mouse monoclonal) | BioLegend | Cat#: 201518 | FACS (2.5 ul/test) |
| Antibody | Anti-rat RT1B-AF647 (Mouse monoclonal) | BD Biosciences | Cat#: 562223 | FACS (1.25 ul/test) |
| Antibody | Anti-rat CD45RA-BV421 (Mouse monoclonal) | BD Biosciences | Cat#: 740043 | FACS (1.25 ul/test) |
| Antibody | Anti-rat CD45-BV605 (Mouse monoclonal) | BD Biosciences | Cat#: 740371 | FACS (1.25 ul/test) |
| Sequence-based reagent | Immunoprep Reagent System | Beckman Coulter | Cat#: 7546999 | |
| Sequence-based reagent | AMPure XP | Beckman Coulter | CAT#:A63880 | RRBS (variable) |
| Commercial assay or kit | TruSeq Barcoded Adapters | Illumina | CAT#:FC-121-1003 | RRBS |
| Software, algorithm | CutAdapt | v2.4 | RBBS adapter trimming | |
| Software, algorithm | BSSeeker2 | v2.0.4 | RRBS Alignment / Methylation Calling |