Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) |
shot3 | Bloomington Drosophila Stock Center | BSDC:2282 FBst0005141 |
Lee et al., 2000 |
| Genetic reagent (D. melanogaster) |
shotkakP2 | Bloomington Drosophila Stock Center | BSDC:29034 FBst0029034 |
Gregory and Brown, 1998 |
| Genetic reagent (D. melanogaster) |
shotΔEGC | F. Jankovics | - | Takács et al., 2017 |
| Genetic reagent (D. melanogaster) |
Rca1G012 | S.J. Araújo | - | Ricolo et al., 2016 |
| Genetic reagent (D. melanogaster) |
tau[MR22] | Bloomington Drosophila Stock Center | BDSC:9530 FBst0009530 |
Doerflinger et al., 2003 |
| Genetic reagent (D. melanogaster) |
bs0326 | BSDC:83157 FBst0083157 |
Guillemin et al., 1996 | |
| Genetic reagent (D. melanogaster) |
btl::moeRFP | M. Affolter | - | Ribeiro et al., 2004 |
| Genetic reagent (D. melanogaster) |
btl-Gal4 | M. Affolter | - | - |
| Genetic reagent (D. melanogaster) |
DSRF4X-Gal4 | A.Ghabrial M. Metzstein |
- | - |
| Genetic reagent (D. melanogaster) |
UAS-shot L(A) GFP | Bloomington Drosophila Stock Center | BDSC:29044 (FBst0029044) |
Lee and Kolodziej, 2002a |
| Genetic reagent (D. melanogaster) |
UAS-shot L(C)-GFP | Bloomington Stock Center | BDSC:29042 FBst0029042 |
Lee and Kolodziej, 2002a |
| Genetic reagent (D. melanogaster) |
UAS-shot L(C)-GFP | Bloomington Stock Center | BDSC:29043 FBst0029043 |
Lee and Kolodziej, 2002a |
| Genetic reagent (D. melanogaster) |
UAS-shot-LA-ΔCtail-GFP | N. Sanchez-Soriano | - | Alves-Silva et al., 2012 |
| Genetic reagent (D. melanogaster) |
UAS-shot-LA-Ctail-GFP | N. Sanchez-Soriano | - | Alves-Silva et al., 2012 |
| Genetic reagent (D. melanogaster) |
UAS-TauGFP | M. Llimargas | - | Llimargas et al., 2004 |
| Genetic reagent (D. melanogaster) |
UAS-bazooka YFP | J. Casanova | - | Gervais and Casanova, 2010 |
| Genetic reagent (D. melanogaster) |
UAS-srcGFP | Bloomington Stock Center | BDSC 5432 FBti0013990 |
Kaltschmidt et al., 2000 |
| Genetic reagent (D. melanogaster) |
UAS-shot RNAi | Bloomington Stock Center | BSSC_64041 FBst0064041 |
Perkins et al., 2015 |
| Genetic reagent (D. melanogaster) |
UAS-lifeActRFP | Bloomington Stock Center | BDSC:58715 FBti0164961 |
- |
| Genetic reagent (D. melanogaster) |
shot::GFP | J.Pastor-Pareja | - | Sun et al., 2019 |
| Antibody | mouse anti GASP | DSHB |
ID:
AB_528492
2A12 |
1:5 |
| Antibody | rat anti DE-cad | DSHB |
ID:AB528120
DCAD2 |
1:100 |
| Antibody | guinea Pig anti CP309 | V. Brodu | - | 1:1000 |
| Antibody | rabbit and rat anti DSRF | J. Casanova | - | 1:500 |
| Antibody | goat anti-GFP | Abcam | Catalog # AB6673 | 1:500 |
| Antibody | rabbit anti-GFP | Invitrogen | Catalog # A11122 | 1:500 |
| Antibody | chicken anti-βgal | Abcam | Catalog # AB134435 | 1:500 |
| Antibody | mouse anti-βgal | Promega | Catalog # 23783 | 1:500 |
| Antibody | mouse antiacetylated tubulin | Millipore | Catalog # 3408 | 1:100 |
| Antibody | guinea pig anti Shot | K. Röper | - | 1:1000 |
| Antibody | mouse anti-Tau-1 | Sigma-Aldrich |
Catalog # MAB3420 Clone PC1C6 |
1:200 |
| Antibody | mouse anti-Actin | MP Biomedicals | Catalog # 691001 | 1:500 |
| Antibody | anti-dig POD fragments | Roche | Catalog # 11 207 733 910 | 1:1000 |
| Antibody | Goat Anti-Mouse Cy3 (Polyclonal) |
Jackson ImmunoResearch |
Catalog # 115-165-003 | 1:500 |
| Antibody | Goat anti-Mouse Alexa555 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-21424 | 1:500 |
| Antibody | Goat anti-mouse Alexa488 (Polyclonal) | LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A11029 | 1:500 |
| Antibody | Donkey anti mouse Alexa647 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A31571 | 1:500 |
| Antibody | Goat anti-chicken Alexa555 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-21437 | 1:500 |
| Antibody | Goat anti-chicken Alexa488 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-11039 | 1:500 |
| Antibody | Goat anti-chicken Alexa Fluor 647 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-21449 | 1:500 |
| Antibody | Goat anti-rabbit Alexa 555 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-21429 | 1:500 |
| Antibody | Goat anti-rabbit Alexa488 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A11008 | 1:500 |
| Antibody | goat anti-rabbit Alexa647 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-21244 | 1:500 |
| Antibody | Goat anti-guinea pig Cy2 (Polyclonal) |
Jackson ImmunoResearch |
Catalog # 706-225-148 | 1:500 |
| Antibody | Goat anti-guinea pig 647 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-21244 | 1:500 |
| Antibody | Donkey anti- mouse Alexa 555 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A31570 | 1:500 |
| Antibody | Donkey anti- mouse Alexa488 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-21202 | 1:500 |
| Antibody | Donkey anti- mouse Alexa647 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A31571 | 1:500 |
| Antibody | Donkey anti- rabbit Alexa555 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A31572 | 1:500 |
| Antibody | Donkey anti- rabbit Alexa488 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-21206 | 1:500 |
| Antibody | Donkey anti- rabbit Alexa647 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog #A31573 | 1:500 |
| Antibody | Donkey anti-goat Alexa 555 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog #A21432 | 1:500 |
| Antibody | Donkey anti-goat Alexa 488 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A32814 | 1:500 |
| Antibody | Donkey anti-rat Cy3 (Polyclonal) |
Jackson ImmunoResearch |
Catalog #712-165-150 | 1:500 |
| Antibody | Goat Anti-rat Alexa 488 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A11006 | 1:500 |
| Antibody | Goat anti-Rat Alexa Fluor647 (Polyclonal) |
LIFE TECHNOLOGIES/Thermofisher Scientific | Catalog # A-21247 | 1:500 |
| Antibody | Biotinylated goat anti-mouse IgM (Polyclonal) |
Thermo Fischer Scientific | Catalog #31804 | 1:500 |
| Sequence-based reagent | Shot mRNA probe | This work | - | 1:100 |
| Other | CBP | J. Casanova | - | 1:500 |
| Other | Fluostain | Sigma-Aldrich | FB28 | 1:300 |
| Other | Vectastain-ABC kit | Vector Laboratories |
PKU-400 | 1:200 |
| Other | TSA Cy3 | Akoya Bio | NEL744001KT | 1:100 |