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. 2020 Oct 26;9:e55865. doi: 10.7554/eLife.55865

Figure 2. Increased expression of ER-stress markers in mice with HCC.

(A) mRNA expression of ER-stress markers Edem1, Ero1b, Grp94, Herp, Atf4, Eif2ak3, Ddit3, and Hspa5 in liver tissue from healthy mice; and tumor tissue and surrounding non-tumoral tissue from mice with DEN-induced HCC. (B) Hspa5-mRNA and (C) protein expression of BIP in murine liver tissue. (D) Ratio of spliced to unspliced XBP1 in liver tissue from healthy mice; and tumor tissue and surrounding non-tumoral tissue from mice with DEN-induced HCC, treated with 4μ8C. (E) Representative western blot image of spliced and unspliced XBP1 protein and vinculin in healthy liver, DEN-induced HCC and DEN-induced HCC treated with 4μ8C. (F) quantification of spliced and unspliced XBP1, normalized to total vinculin levels. (G) Ratio of spliced to unspliced XBP1 protein levels. (H) Representative images and (I) quantification of liver tissue sections stained with antibodies against spliced XBP1. p-Values were calculated via the Student's T-test with five biological replicates per group. Scale bars = 120 μm.

Figure 2.

Figure 2—figure supplement 1. Activation of the unfolded protein response is mainly located in the stroma of mice with HCC.

Figure 2—figure supplement 1.

Liver tissue from mice with DEN-induced HCC, stained with αSMA-antibodies and co-stained with antibodies against (A) spliced XBP1, (B) total XBP1, (C) IRE1α (D) phopho-IRE1α, and (E) BIP. Scale bars = 50 μm.
Figure 2—figure supplement 2. Expression of ER-stress markers is localized in close vicinity to αSMA.

Figure 2—figure supplement 2.

Immunofluorescent images from tissue from mice with DEN-induced HCC, stained with αSMA-antibodies and co-stained with antibodies against (A) spliced XBP1, (B) total XBP1, (C) IRE1α, (D) phopho-IRE1α, and (E) BIP. (F) Immunofluorescent image from DEN-induced HCC stained with antibodies against spliced XBP1.