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. 2020 Oct 26;9:e55865. doi: 10.7554/eLife.55865

Figure 5. Inhibiting IRE1α decreases stellate cell activation in human liver 3D scaffolds engrafted with stellate cells and tumor cells.

Figure 5.

(A) Representative images of H and E and Sirius red stained slides of decellularized human liver scaffolds engrafted with LX2 stellate cells and HepG2-tumor cells treated with 4μ8C or control. (B) Quantification of collagen-stained area fraction of liver scaffolds engrafted with LX2 stellate cells and HepG2-tumor cells treated with 4μ8C or control. (C) mRNA-expression of the stellate cells activation marker collagen and ER-stress markers HSPA5, spliced XBP-1 (XBP1-S), and DDIT3 in liver scaffolds engrafted with stellate cells (LX2) and cancer cells (HepG2), treated with 4μ8C or control. p-Values were calculated via ANOVA from three biological replicates per group, scale bars = 100 μm.