| Strain, strain background (Mus musculus)
|
Sv129 mice |
Taconic |
129S6 |
HCC mouse model, Heindryckx et al., 2010; Heindryckx et al., 2012
|
| Cell line (Homo sapiens) |
HepG2 |
ATCC |
HB-8065 |
|
| Cell line (Homo sapiens) |
Huh7 |
Gifted, Karolinska institute |
|
|
| Cell line (Homo sapiens) |
LX2 |
Sigma-Aldrich |
SCC064 |
|
| Transfected construct (human) |
si-IRE1α |
ThermoFisher |
s200432 |
0,1–1 µM |
| Transfected construct (human) |
Si-Ctrl; Scr |
ThermoFisher |
4390843 |
0,1–1 µM |
| Antibody |
KI67 (rat monoclonal) |
eBioscience |
SolA15 |
1:100 |
| Antibody |
EPCAM (rabbit polyclonal) |
Abcam |
ab71916 |
1:100 |
| Antibody |
Spliced XBP1 (goat monoclonal) |
Abcam |
Ab85546 |
1:50 |
| Antibody |
Total XBP1 (Rabbit polyclonal) |
Abcam |
Ab37152 |
5 µg/ml |
| Antibody |
IRE1a (rabbit polyclonal) |
Abcam |
Ab37073 |
1 µg/ml |
| Antibody |
p-IRE1 (rabbit polyclonal) |
AbNova |
PAB12435 |
1:100 |
| Antibody |
αSMA (Rabbit Polyclonal) |
ThermoFisher |
710487 |
1:200 |
| Antibody |
αSMA (Goat monocolonal) |
Abcam |
Ab21027 |
1–2 µg/ml |
| Antibody |
BIP (goat polyclonal) |
Abcam |
Ab21027 |
1 µg/ml |
| Antibody |
Vinculin (Mouse monoclonal) |
ThermoFisher |
14-9777-82 |
1–5 µg/ml |
| Peptide, recombinant protein |
Pst-I |
ThermoFisher |
ER0615 |
|
| Commercial assay or kit |
Pierce BCA-protein assay kit |
ThermoFisher |
233225 |
|
| Commercial assay or kit |
EZNA RNA isolation Kit II |
VWR |
R6934-02 |
|
| Commercial assay or kit |
RNeasy Universal Mini Kit |
Qiagen |
73404 |
|
| Commercial assay or kit |
Diva Decloacker solution |
Biocare |
DV2004 |
|
| Commercial assay or kit |
DCFDA - Cellular ROS Detection Assay Kit |
Abcam |
ab113851 |
|
| Chemical compound, drug |
N-Nitrosodiethylamine, DEN |
Sigma-Aldrich |
1002877809 |
|
| Chemical compound, drug |
4μ8C |
Sigma-Aldrich |
SML0949-25MG |
Heindryckx et al., 2016 |
| Chemical compound, drug |
SB-431541, TGF-ß receptor inhibitor |
Tocris |
1614 |
10 μM |
| Chemical compound, drug |
Resazurin |
Sigma-Aldrich |
R7017-1G |
1:80 dilution |
| Commercial assay or kit |
Ingenio electroporation solution |
Mirus Bio LLC |
MIR50114 |
Ice-cold |
| Commercial assay or kit |
CellTracker Red CMTPX |
ThermoFisher |
C34552 |
1 μM |
| Commercial assay or kit |
CellTracker Green CMFDA |
ThermoFisher |
C2925 |
1 μM |
| Other |
12-well CorningCostar Transwellplates |
Sigma-Aldrich |
3460 |
Calitz et al., 2020 |
| Other |
Corning CostarUltra-Low attachment 96-well plates |
Sigma-Aldrich |
CLS3471 |
Calitz et al., 2019 |
| Other |
CellDirector |
GradienTech |
11-001-10 |
Fuchs et al., 2020 |
