Table 4. Primers of qPCR used in the experiment.
Genes | Sequences (5ʹ - 3ʹ) | Product size | Tm (°C) | |
---|---|---|---|---|
Heat shock protein kDa70, HSP70 | F: AAGGCAGAGGATGATGTC | 186 | 59 | Mohd-Shaharuddin, Mohd-Adnan [48] |
R: TGCAGTCTGGTTCTTGTC | ||||
Heat shock protein kDa90, HSP90 | F: ACCTCCCTCACAGAATACC | 197 | 59 | Mohd-Shaharuddin, Mohd-Adnan [48] |
R: CTCTTGCCATCAAACTCC | ||||
18S rRNA, 18S | F: TGGTTAATTCCGATAACGAACGA | 94 | 59/60 | Mohd-Shaharuddin, Mohd-Adnan [48] |
R: CGCCACTTGTCCCTCTAAGAA | ||||
Elongation factor-1α, ef1α | F: AAATTGGCGGTATTGGAAC | 83 | 59/60 | Mohd-Shaharuddin, Mohd-Adnan [48] |
R: GGGAGCAAAGGTGACGAC |