| Antibodies |
|
|
| HA Epitope Tag Antibody (2–2.2.14) |
Thermo Fisher Scientific |
Cat# 26183; RRID: AB_10978021 |
| beta-actin (13E5) rabbit mAB |
Cell Signaling Technology |
Cat# 4970S; RRID: AB_2223172 |
| Rat monoclonal antibody to DYKDDDDK eptiope tag |
Agilent |
Cat# 200474; RRID: AB_10597743 |
| Anti-mouse IgG, HRP-linked Antibody |
Cell Signaling Technology |
Cat# 7076S |
| RRID: AB_330924 |
| Anti-rabbit IgG, HRP-linked Antibody |
Cell Signaling Technology |
Cat# 7074S |
| RRID: AB_2099233 |
| Anti-rat IgG, HRP-linked Antibody |
Cell Signaling Technology |
Cat# 7077S |
| RRID: AB_10694715 |
| Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 |
Thermo Fisher Scientific |
Cat# A-11004, RRID: AB_2534072 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Dulbecco’s Modified Eagle Medium (DMEM) |
Thermo Fisher Scientific,Invitrogen |
Cat# 11965092 |
| Antibiotic-Antimycotic (100X) |
Thermo Fisher Scientific,Invitrogen |
Cat# 15240062 |
| FreeStyle 293 Expression Medium |
Thermo Fisher Scientific,Invitrogen |
Cat# 12338026 |
| Geneticin Selective Antibiotic (G418 Sulfate), Powder |
Thermo Fisher Scientific,Invitrogen |
Cat# 11811031 |
| Corning BioCoat Poly-D-Lysine |
Corning |
Cat# 354210 |
| Chelex® 100 sodium form |
Sigma-Aldrich |
Cat# C7901-25G |
| N,N,N′,N′-Tetrakis(2-pyridylmethyl) ethylenediamine (TPEN) |
Sigma-Aldrich |
Cat# P4413 |
| Dulbecco’s Phosphate Buffered Saline |
Sigma-Aldrich |
Cat# D8537-500ML |
| Immobilon-P PVDF Membrane |
Millipore |
Cat# PVH00010
|
| Transferrin From Human Serum, Alexa Fluor 488 Conjugate |
Thermo Fisher Scientific |
Cat# T13342
|
| Normal Goat Serum |
Cell Signaling Technology |
Cat# 5425S |
| Mounting Medium With DAPI - Aqueous, Fluoroshield |
Abcam |
Cat# ab104139 |
| Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K) |
Polysciences |
Cat# 23966 |
| ANTI-FLAG® M2 Affinity Gel |
Sigma-Aldrich |
Cat# A2220 |
| Sigma FASTä Protease Inhibitor Cocktail Tablet, EDTA Free |
Sigma-Aldrich |
Cat# S8830 |
| FLAG® Peptide |
Sigma-Aldrich |
Cat# F3290-4MG |
| n-Dodecyl-β-D-Maltopyranoside, Anagrade (DDM) |
Anatrace |
Cat# 69227-93-6 |
| phenylmethylsulfonyl fluoride (PMSF) |
Thermo Fisher Scientific |
Cat# 36978 |
| Critical Commercial Assays |
|
|
| PfuTurbo DNA Polymerase |
Agilent |
Cat# 600250 |
| Wizard® Plus SV Minipreps DNA Purification |
Promega |
Cat# A1460 |
| QIAGEN Plasmid Maxi Kit |
QIAGEN |
Cat# 12163 |
| Lipofectamine 2000 Transfection Reagent |
Thermo Fisher Scientific,Invitrogen |
Cat# 11668019 |
| ECL Prime Western Blotting Detection Reagent |
VWR |
Cat# RPN2232 |
| Deposited Data |
|
|
| Original western blots |
This paper |
Mendeley (http://data.mendeley.com/drafts) |
| Experimental Models: Cell Lines |
|
|
| HEK293 |
ATCC |
Cat# CRL-1573, RRID:CVCL_0045 |
| HEK293T |
ATCC |
Cat# CRL-3216, RRID:CVCL_0063 |
| Freestyle 293-F cells |
Thermo Fisher Scientific |
SKU# R790-07 |
| Recombinant DNA |
|
|
| pEGFP-N1 |
Clonetech |
Cat# 6085-1 |
| Software and Algorithms |
|
|
| ImageJ |
NIH |
RRID: SCR_003070 |
| GraphPad Prism 5 |
GraphPad Prism |
RRID: SCR_002798 |
