Table 1.
Primary information of genotyped polymorphisms in TLR2, 3, 4, and 7 genes.
| Gene | SNPs | Base change | Localization | Primers | Enzyme |
|---|---|---|---|---|---|
| TLR2 | rs5743703/04/05/06/07/09 | G/A-C/A-T/C-T/A-T/G-G/A | Exon 1 | F: 5′-GGCCAGCAAATTACCTGTGT-3′ R: 5′-GATCCCAACTAGACAAAGAC-3′ |
┼ |
| rs5743708 | +2477 G/A (Arg753Gln) | Exon 1 | F: 5′-GGCCAGCAAATTACCTGTGT-3′ R: 5′-GATCCCAACTAGACAAAGAC-3′ |
Pst1 | |
| (GT)n | — | Intron 2 | F: 5′-TATCCCCATTCATTCGTTCCATT-3′ R:5′Hex-GACCCCCAAGACCCACACC-3′ |
$ | |
| TLR3 | rs5743305 | -8441 T/A | Promotor | F: 5′-GGGACAGTCACGTACTCAGGA-3′ R:5′-GTGGGCTCCAGCTTCAACTA-3′ |
SpeI |
| rs3775294 | T/C | Intron 2 | F: 5′-CACATGGCTTATCAAACACAC-3′ R:5′-CAAAGGGTGATAAAAACATCCT-3′ |
Pst1 | |
| rs3775291 | +1234 G/A (Leu412Phe) | Exon 4 | F: 5′-ATCAGTCGTTGAAGGCTTGG-3′ R: 5′-TGCTCATTCTCCCTTACACAGA-3′ |
Tfi1 | |
| TLR4 | rs4986790 | +896A/G (Asp299Gly) | Exon 4 | F: 5′-GATTAGCATACTTAGACTACTACCTCCATG-3′ R: 5′-GATCAACTTCTGAAAAAGCATTCCCAC-3′ |
Nco I |
| rs4986791 | +1196 C/T (Thr399Ile) | Exon 4 | F: 5′-GGTTGCTGTTC CAAAGTGATTTTGGGACAA-3′ R: 5′-GGAAATCCAGATGTTCTAGTTGTTCTAAGCC-3′ |
Hinf1 | |
| TLR7 | rs3853839 | C/G | 3′UTR | F: 5′TTGCTTCCGTGTCATCCAGG3′ R: 5′ACAGTACTTTGCAGTGCAGATAAA3′ |
StyI |
┼: SNPs were genotyped by direct sequencing; $: primers as described previously [24] and was genotyped using the automatic genotyping method.