Table 1.

Sequencing statistics. H25, H26 and H26 are the three different horse’s DNA analysed. Barcode01 to Barcode09 are the names of the barcodes used in the multiplexing with the kit EXP-NBD-104. The category Unclassified refers to reads that were filtered out at the demultiplexing steps because the barcode sequence was not attributed. exo -: no Exonuclease V treatment; exo A: Exonuclease V 10 U 2 h + REPLI-g 0.2 μM primers; exo B: Exonuclease V 10 U 2 h + REPLI-g 1.0 μM primers

Sample	H25 exo -	H26 exo -	H27 exo -	H25 exo A	H26 exo A	H27 exo A	H25 exo B	H26 exo B	H27 exo B
Barcode	Barcode01	Barcode02	Barcode03	Barcode04	Barcode05	Barcode06	Barcode07	Barcode08	Barcode09	Unclassified	Total
Mean read length (nt)	2209	2248	2004	2733	2526	2553	1821	3241	2869	2145	2379
Median read length (nt)	1706	1763	1579	1918	1815	1865	1440	2336	2172	1545	1741
Read length N50 (nt)	2786	2807	2462	3867	3409	3414	2172	4635	3873	2909	3137
Number of reads	60,619	67,448	60,729	59,028	69,453	55,892	88,175	45,641	55,996	71,073	634,054
% of Total reads	10%	11%	10%	9%	11%	9%	14%	7%	9%	11%	100%
Total bases (nt)	133,906,674	151,599,799	121,723,567	161,303,032	175,444,343	142,699,042	160,526,846	147,900,066	160,661,882	152,461,373	1,508,226,624
% of Total bases	9%	10%	8%	11%	12%	9%	11%	10%	11%	10%	100%
Mean read quality	11.5	11.5	11.5	10.9	10.9	10.9	11.3	11.4	11.5	6.8	10.8
Median read quality	11.7	11.8	11.7	11.1	11.2	11.1	11.6	11.7	11.8	5.8	11.3