Figure 1.

Comparison of the effects of pre-exposure to polystyrene microplastic spheres and the field habitat on which they were subsequently placed (live or dead coral patch) on the behaviour of juvenile Pomacentrus amboinensis. The behavioural index is a composite variable (mean ± s.e.), represented as PC1 of a principal component analysis on bite rate, total distance moved in 3 min, maximum distance ventured from shelter and a boldness index (see text). As represented, the behavioural index is positively related to all variables used in the analysis. All variables were strongly correlated with PC1 (see text). Letters represent Tukey's HSD means comparisons groupings. N (left to right) = 28, 32, 32, 31. (Online version in colour.)