Table 3.
Microbial alkane gas production via amino acid biosynthesis and/or degradation routes
| Alkane production (mg/L) | ||||
|---|---|---|---|---|
| Terminal Enzyme | Propane | Butane | Isobutane | Comments |
| KdcA-dependent pathway in E. coli (valine biosynthesis) [10] | ||||
| ADOWT | 0.091 | na | na | E. coli BW25113(DE3)Δ13 strain |
| ADOI127G | 0.267 | na | na | E. coli BW25113(DE3)Δ13 strain |
| KdcA-dependent pathway in E. coli (amino acid catabolism) [5, 6] | ||||
| CvFAPG462I | 7.8 | 7.6 | 48.7 | Endogenous amino acids |
| CvFAPG462I | 109.7 | 142 | 112.1 | 30 mM amino acids supplied |
| KdcA-dependent pathway in Halomonas (amino acid catabolism) with added amino acids [5, 6] | ||||
| CvFAPG462I | 8.0 | 0.11 | 0.65 | Plasmid-borne construct |
| CvFAPG462I | 2.7 | 0.04 | 0.29 | Genomic integrated construct |
|
CoA-dependent pathways in E. coli (amino acid catabolism) [5] | ||||
| ADOA134F | 0.96 | 0.03 | 0.01 | Endogenous amino acids |
| CvFAPG462I | 0.98 | 0.09 | 0.04 | Endogenous amino acids |
In vivo studies determined in the presence or absence of supplemental amino acids (30 mM)
na not applicable