Figure 2.

Expression of immune checkpoint receptors and ligands in the tumor samples of human OVC patients. (A) Expression of coinhibitory checkpoint molecules on the CD8 T+cell surface (PD-1, LAG3, TIM-3, CTLA-4 and TIGIT) in freshly thawed PBMC (blue dots) and TILs/TALs (red square). (B) Expression of checkpoint ligands in PBMC (blue dots) and EpCAM+ or EpCAM- (CD80, CD86) TILs/TALs (red square). Mann-Whitney U test: *P<0.05; **p<0.005; ***p<0.001. (C) The relationship between the level of coinhibitory checkpoint molecules on TILs/TALs and disease stages. Correlation, R²=0.91, *p<0.05. (D) The relationship between the level of cognate ligands in tumor samples and disease stages. Correlation, R²=0.99, **p<0.005. OVC, ovarian cancer; PBMC, peripheral blood mononuclear cell; TALs, tumor-associated leukocytes; TIL, tumor-infiltrating leukocyte.