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. 2020 Oct 30;11:568887. doi: 10.3389/fpls.2020.568887

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Copyright © 2020 Lin, Gao, Zhao, Zhang, Chen and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expressing SaPCR2 enhanced cadmium (Cd) tolerance in yeast mutant Δzrc1 by reducing Cd concentration. (A) The yeast strain BY4743 and Δzrc1 transformed with pDR196-SaPCR2 vector or pDR196 empty vector (EV) were diluted (OD₆₀₀ = 1.0, 0.1, 0.01, and 0.001) and spotted on SD medium with 0, 10, and 60 μM CdCl₂ for 3 days at 30°C. (B) Cd concentration in yeasts. Δzrc1 strains expressed EV and pDR196-SaPCR2 were cultured in SD liquid medium with 2.5, 5, and 10 μM CdCl₂ for 24 h at 30°C. Error bars are ± SE of n = 4. ^∗Indicates significant differences between Δzrc1-EV and Δzrc1-SaPCR2 (p < 0.05).