Fig. 4.

Histochemistry and fluorescence of glandular trichomes of Hartweg's locust – lipophilic compounds. a–d, g, h – LM. e, f, i–l—FM. a, b Staining of total lipids with Sudan Red B. c, d Staining of total lipids with Sudan IV. e, f Fluorescence of lipids with the Auramine O fluorochrome in the Cy 5 filter set. g, h Staining of neutral and acidic lipids with Nile Blue. i, j Fluorescence of lipids with the Auramine O fluorochrome in the Cy5 (i) and TRITC (j) filter sets. k, l Fluorescence of steroid-containing terpenes with the antimony trichloride fluorochrome in the Cy5 (k) and TRITC (l) filter sets. Scale bars = 100 µm (a–c, e–k), 50 µm (d, l)