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. 2020 Oct 30;8:586541. doi: 10.3389/fcell.2020.586541

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Copyright © 2020 He, Liu, Huang, Zhuo, Chen, Duan, Tang, Lu and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Olfactory mucosa mesenchymal stem cells (OM-MSCs) upregulated SPCA1 expression in oxygen and glucose deprivation/reoxygenation (OGD/R)-treated N2a cells and ischemia/reperfusion (I/R) rats. (A–C) SPCA1 messenger RNA (mRNA) and protein expressions of OGD/R-treated N2a cells at different time points were visualized by quantitative PCR (qPCR) and Western blot. (D–F) SPCA1 mRNA and protein expressions of N2a cells in the normal, OGD4h/R24h, and OM-MSC co-culture groups were measured by qPCR and Western blot. (G–I) SPCA1 mRNA and protein expressions of rats’ ipsilateral brain samples in the sham, I/R, and OM-MSC transplantation groups were detected by qPCR and Western blot. Data are shown as the mean ± SEM based on three independent experiments. *p ≤ 0.05, compared with the normal or sham group and ^&p ≤ 0.05, compared with the OGD4h/R24h or I/R group.