FIGURE 7.

Olfactory mucosa mesenchymal stem cells (OM-MSCs) attenuated Ca²⁺ overload and improved Golgi apparatus (GA) morphology in oxygen and glucose deprivation/reoxygenation (OGD/R)-treated N2a cells and ischemia/reperfusion (I/R) rats. (A,B) Ca²⁺ concentrations in the cytoplasm and GA of OGD/R-treated N2a cells at different time points were determined by the Ca²⁺ Assay Kit. (C,D) Ca²⁺ concentrations in the cytoplasm and GA of N2a cells in the normal, OGD4h/R24h, and OM-MSC co-culture groups were measured by the Ca²⁺ Assay Kit. (E,F) Intracellular Ca²⁺ of rats’ ipsilateral brain samples in the sham, I/R, and OM-MSC transplantation groups were detected by flow cytometry analysis using a Fluo-3/AM kit. (G) Representative image of GA ultramicrostructure changes by using an electron microscope (scale bar, 2 μm). The GA was indicated by the magenta arrow. Data are shown as the mean ± SEM based on three independent experiments. *p ≤ 0.05, compared with the normal or sham group and ^&p ≤ 0.05, compared with the OGD4h/R24h or I/R group.