Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 30;8:586541. doi: 10.3389/fcell.2020.586541

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 He, Liu, Huang, Zhuo, Chen, Duan, Tang, Lu and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Olfactory mucosa mesenchymal stem cells (OM-MSCs) protected N2a cells from oxygen and glucose deprivation/reoxygenation (OGD/R)-induced injury through modulating SPCA1. (A–C) SPCA1 messenger RNA (mRNA) and protein expressions in both the control short hairpin RNA (shRNA) group and the SPCA1 shRNA group were visualized by quantitative PCR (qPCR) and Western blot. (D,E) Apoptosis in both the control shRNA group and the SPCA1 shRNA group was evaluated by flow cytometry analysis. (F,G) Intracellular reactive oxygen species (ROS) in both the control shRNA group and the SPCA1 shRNA group was detected using an oxidation-sensitive fluorescent probe (DCFH-DA). (H) Cell death in both the control shRNA group and the SPCA1 shRNA group was determined by the lactate dehydrogenase (LDH) assay. (I,J) Ca²⁺ concentrations in the cytoplasm (I) and the Golgi apparatus (GA; J) in both the control shRNA group and the SPCA1 shRNA group were determined by the Ca²⁺ Assay Kit. Data are shown as the mean ± SD based on three independent experiments. *p ≤ 0.05, compared with the normal group; ^&p ≤ 0.05, compared with the OGD4h/R24h group; and ^#p ≤ 0.05, compared with the control shRNA group. ^$p ≤ 0.05, ns. p > 0.05, compared with OGD4h/R24h of the control shRNA group.