Figure 1.
Lesion area (%) of acibenzolar-S-methyl (ASM)-treated and untreated leaves of Japanese radish after Pseudomonas cannabina pv. alisalensis (Pcal) inoculation. Greenhouse-grown Japanese radish plants were spray-inoculated with Pcal (5 × 107 CFU/ml) 4 h (A), 1 day (B), and 1 week (C) after ASM dip-treatment (100 ppm) on fourth leaves. Disease symptoms of the ASM-treated fourth leaves and untreated upper (fifth) and lower (third) leaves were monitored by measuring lesion areas at 1-week post inoculation (wpi). Vertical bars indicate the SE for four independent replications. Asterisks indicate a significant difference from the water treatment control in a t-test (*p < 0.05; **p < 0.01).