Figure 2.
Bacterial populations of ASM-treated and untreated systemic leaves of Japanese radish after Pcal inoculation. Three-week-old Japanese radish plants were spray-inoculated with Pcal suspensions (5 × 107 CFU/ml) 4 h (A), 1 day (B), and 1 week (C) after ASM dip-treatment (100 ppm) on fourth leaves. Bacterial populations in the ASM-treated fourth leaves and untreated systemic upper (fifth) and lower (third) leaves were determined by dilution plating on selective medium as described in the methods section at 1-wpi. Vertical bars indicate the SE for three independent experiments. Asterisks indicate a significant difference from the water treatment control in a t-test (*p < 0.05).