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. 2020 Oct 30;11:565745. doi: 10.3389/fpls.2020.565745

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Copyright © 2020 Sakata, Ishiga, Taniguchi and Ishiga.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stomatal aperture width (μm) in ASM-treated Japanese radish plants after application with SHAM or DPI. Fourth leaves of Japanese radish were dip-treated with ASM (100 ppm) and after 1 week, SHAM (1 mM), and DPI (10 μM) was dip-treated to the ASM-treated fourth leaves, and the untreated systemic upper (fifth) and lower (third) leaves. The microscope images of third (A), fourth (B), and fifth (C) leaves were taken 4 h after treatment of the chemicals using a Nikon optical microscope (Eclipse 80i). Then, stomatal aperture width (μm) were analyzed by image J using at least 100 stomata. In all bar graphs, vertical bars indicate the SE for three biological replicates. Significant differences (p < 0.05) are indicated by different letters based on a Tukey’s HSD test.