TABLE 2.
Summary table showing the minimum and maximum (Min_Max) number of total and filtered reads analyzed per group and the minimum and maximum proportion (%) of reads per group which align against OTUs classified into a particular taxonomic category.
Country | Host | Year | T | Fix. | N° | Total reads | Filtered reads | Minimum and Maximum proportion of reads |
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ELO | Anaplasma | Myco | Franc | Other | Envirom | Unclass | ||||||||
Japan | Mizuhopecten yessoensis | 2017 | DG | EtOH | 7 | 1890_124065 | 184_22374 | 2_84 | 0_77 | 0 | 0 | 0 | 0.1_57 | 11_39 |
G | 7 | 190215_518086 | 42533_128106 | 53_89 | 0_2 | 0 | 0 | 0_0.004 | 0.06_0.3 | 10_46 | ||||
New Zealand | Pecten novaezelandiae | 2015 | G, M | EtOH | 2 | 201879_265388 | 152113_191950 | 33_39 | 0.007_0.009 | 0_0.006 | 0 | 0_0.002 | 2_21 | 45_57 |
V | FFPE | 1 | 15831 | 7721 | 9 | 0.2 | 0.05 | 0 | 0 | 31 | 59 | |||
2017 | G | EtOH | 7 | 61504_497640 | 12554_108335 | 47_67 | 0_0.02 | 0 | 0 | 0_0.02 | 0.04_0.5 | 33_53 | ||
Ostrea chilensis | 2014 | G | EtOH | 2 | 46976_161146 | 36563_84196 | 4_35 | 0_0.3 | 0.05_0.3 | 0.2_56 | 0 | 2_7 | 36_57 | |
Paphies ventricosa | 2017 | G, M | EtOH | 3 | 97852_402102 | 13639_69775 | 0.2_8 | 0.003_0.01 | 0 | 0 | 1_8 | 49_73 | 25_42 | |
Panopea zelandica | 2014 | G | EtOH | 2 | 119124_200755 | 80651_128890 | 0_1 | 0_0.1 | 0 | 0 | 0.09_21 | 22_52 | 24_77 | |
Perna canaliculus | 2014 | G | EtOH | 2 | 83306_309316 | 57205_145883 | 6_7 | 0 | 0.62_7.4 | 0.007_0.01 | 0 | 66_80 | 12_19 | |
South Africa | Pecten sulcicostatus | 2010/11 | V | FFPE | 4 | 213_6092 | 35_1027 | 63_88 | 0 | 0_0.19 | 0 | 0 | 6–17 | 4_17 |
United Kingdom | Mytilus edulis | 2013 | DG | EtOH | 4 | 90113_340995 | 67417_259761 | 0.1_0.3 | 0_64 | 0 | 0.7_67 | 0 | 2_47 | 28_56 |
Aequipecten opercularis | 2017 | G | EtOH | 6 | 102788_1127022 | 78011_728073 | 68_98 | 0_0.008 | 0.008_0.4 | 0_0.005 | 0 | 0.3_14 | 1_16 | |
DG | EtOH | 3 | 51328_595336 | 36838_448872 | 10_61 | 0_0.06 | 0.53_5 | 0 | 0_1 | 13_48 | 18_35 | |||
Ostrea edulis | 2016 | DG | EtOH | 4 | 67093_118337 | 880_81236 | 1_76 | 0.02_0.7 | 0_37 | 0_0.6 | 0_18 | 4_30 | 17_50 | |
Cerastoderma edule | 2015 | G | EtOH | 2 | 256176_429593 | 198902_322093 | 67_81 | 0.01_0.02 | 0 | 0_0.002 | 0_1 | 0.8_0.9 | 17_31 | |
Ruditapes philippinarum | 2017 | V | EtOH | 2 | 263257_462412 | 172345_280424 | 0.1_0.7 | 30_46 | 0_0.01 | 0_0.01 | 0 | 8_29 | 40_44 | |
Pecten maximus | 2014 | G | EtOH | 1 | 197625 | 161027 | 99 | 0 | 0.02 | 0.006 | 0 | 0.1 | 0.2 | |
France | Pecten maximus | 2009 | G, DG | EtOH | 2 | 122866_534503 | 80998_418875 | 32_72 | 0 | 0.3_11 | 0_0.005 | 1_3 | 4_7 | 21_47 |
2009 | G | EtOH | 3 | 214283_489903 | 168343_410408 | 84_100 | 0_0.01 | 0.03_0.4 | 0_0.01 | 0.001_0.3 | 0.07_1 | 0.1_13 | ||
Ireland | Mytilus edulis | 2016 | G | EtOH | 1 | 417337 | 298547 | 0.2 | 0.009 | 0.003 | 0 | 0.01 | 91 | 7 |
Ruditapes philippinarum | 2014 | DG | EtOH | 2 | 279012_354851 | 221600_274155 | 55_61 | 0.11_0.15 | 0 | 0_0.01 | 0_0.3 | 0.3_0.4 | 38_44 | |
G | EtOH | 1 | 25194 | 13342 | 2 | 0 | 0 | 0.02 | 0.01 | 68 | 29 | |||
Pecten maximus | 2016 | V | EtOH | 4 | 126068_572994 | 91340_417156 | 0.08_05 | 0 | 0.3_7 | 0_0.01 | 0.6_5 | 49_69 | 28_37 | |
Italy | Mytilus galloprovincialis | 2018 | G | EtOH | 2 | 20620_24086 | 3481_3820 | 59_70 | 0_0.08 | 0 | 0 | 0 | 0.6_1 | 28_40 |
Callista chione | 2011 | V | FFPE | 3 | 3992_26430 | 1606_11407 | 1_29 | 0_1 | 0 | 0_1.6 | 0_13 | 14_48 | 18_55 | |
Donax trunculus | 2018 | V | FFPE | 2 | 8415_13030 | 864_4680 | 6_67 | 0_0.2 | 0 | 0 | 0 | 13_38 | 19_55 | |
Patella caerulea | 2018 | V | FFPE | 1 | 15720 | 9858 | 2 | 0.3 | 0 | 0 | 0 | 19 | 78 | |
Spain | Mytilus galloprovincialis | 2013 | V | EtOH | 2 | 36506_190172 | 17380_142898 | 52_67 | 0_0.01 | 0 | 0_0.01 | 0 | 0.3_1 | 31_47 |
Cerastoderma edule | 2013 | V | FFPE | 1 | 24968 | 2670 | 28 | 0 | 0 | 0 | 0 | 14 | 57 | |
2014 | V | EtOH | 1 | 925235 | 768328 | 90 | 0 | 0 | 0.001 | 0 | 0.009 | 9 | ||
Mimachlamys varia | 2012 | V | EtOH | 2 | 83588_405889 | 56110_279185 | 0_17 | 0_0.05 | 5_8 | 0.003_0.06 | 0 | 13_16 | 60_78 | |
Polititapes aureus | 2010 | V | FFPE | 1 | 2162 | 531 | 31 | 0 | 0 | 0 | 0 | 57 | 10 | |
Norway | Ostrea edulis | 2017 | DG | EtOH | 4 | 84350_268876 | 9646_41698 | 0.1_2 | 32_70 | 0 | 0 | 0_0.01 | 2_16 | 23_51 |
Chile | Mytilus chilensis | 2016 | V | FFPE | 4 | 12087_64271 | 2631_28234 | 2_34 | 0_0.1 | 0_1.5 | 0_0.4 | 0_12 | 5_74 | 18_59 |
Argopecten purpuratus | 2014 | V | FFPE | 2 | 3306_12636 | 1083_1272 | 21_38 | 4_6 | 0_0.4 | 0_3 | 0 | 20_27 | 32_44 |
ELO: Endozoicomonas-like organisms, Anaplasma group. Myco: Mycoplasma group. Franc: Francisella group. Other: shows the minimum and maximum proportion of reads with similarity to other symbionts, grouped either with Chlamydia, Bacteroidetes, Spirochetes or sulfur-oxidizing endosymbiotic bacteria. Envirom: environmental, shows the minimum and maximum proportion of reads that were not identified as symbionts and were then classified as environmental. Unclass: unclassified, shows the minimum and maximum proportion of reads per sample that were not included within the top ten percent of reads in any one sample or which were not identified as the most abundant in any one sample and which were therefore not investigated. T: tissue analyzed as follows: “G” gill; “D” digestive gland; “M” mantle, and “V” various organs (for DNA extracted from FFPE). Fix: DNA extracted from ethanol fixed (EtOH) or from formalin-fixed and paraffin-embedded (FFPE) tissues. N°: number of samples sequenced per group. Details of the metadata associated with each sample can be found in Supplementary Table 1.