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. 2020 Oct 30;11:577481. doi: 10.3389/fmicb.2020.577481

TABLE 2.

Summary table showing the minimum and maximum (Min_Max) number of total and filtered reads analyzed per group and the minimum and maximum proportion (%) of reads per group which align against OTUs classified into a particular taxonomic category.

Country Host Year T Fix. Total reads Filtered reads Minimum and Maximum proportion of reads
ELO Anaplasma Myco Franc Other Envirom Unclass
Japan Mizuhopecten yessoensis 2017 DG EtOH 7 1890_124065 184_22374 2_84 0_77 0 0 0 0.1_57 11_39
G 7 190215_518086 42533_128106 53_89 0_2 0 0 0_0.004 0.06_0.3 10_46
New Zealand Pecten novaezelandiae 2015 G, M EtOH 2 201879_265388 152113_191950 33_39 0.007_0.009 0_0.006 0 0_0.002 2_21 45_57
V FFPE 1 15831 7721 9 0.2 0.05 0 0 31 59
2017 G EtOH 7 61504_497640 12554_108335 47_67 0_0.02 0 0 0_0.02 0.04_0.5 33_53
Ostrea chilensis 2014 G EtOH 2 46976_161146 36563_84196 4_35 0_0.3 0.05_0.3 0.2_56 0 2_7 36_57
Paphies ventricosa 2017 G, M EtOH 3 97852_402102 13639_69775 0.2_8 0.003_0.01 0 0 1_8 49_73 25_42
Panopea zelandica 2014 G EtOH 2 119124_200755 80651_128890 0_1 0_0.1 0 0 0.09_21 22_52 24_77
Perna canaliculus 2014 G EtOH 2 83306_309316 57205_145883 6_7 0 0.62_7.4 0.007_0.01 0 66_80 12_19
South Africa Pecten sulcicostatus 2010/11 V FFPE 4 213_6092 35_1027 63_88 0 0_0.19 0 0 6–17 4_17
United Kingdom Mytilus edulis 2013 DG EtOH 4 90113_340995 67417_259761 0.1_0.3 0_64 0 0.7_67 0 2_47 28_56
Aequipecten opercularis 2017 G EtOH 6 102788_1127022 78011_728073 68_98 0_0.008 0.008_0.4 0_0.005 0 0.3_14 1_16
DG EtOH 3 51328_595336 36838_448872 10_61 0_0.06 0.53_5 0 0_1 13_48 18_35
Ostrea edulis 2016 DG EtOH 4 67093_118337 880_81236 1_76 0.02_0.7 0_37 0_0.6 0_18 4_30 17_50
Cerastoderma edule 2015 G EtOH 2 256176_429593 198902_322093 67_81 0.01_0.02 0 0_0.002 0_1 0.8_0.9 17_31
Ruditapes philippinarum 2017 V EtOH 2 263257_462412 172345_280424 0.1_0.7 30_46 0_0.01 0_0.01 0 8_29 40_44
Pecten maximus 2014 G EtOH 1 197625 161027 99 0 0.02 0.006 0 0.1 0.2
France Pecten maximus 2009 G, DG EtOH 2 122866_534503 80998_418875 32_72 0 0.3_11 0_0.005 1_3 4_7 21_47
2009 G EtOH 3 214283_489903 168343_410408 84_100 0_0.01 0.03_0.4 0_0.01 0.001_0.3 0.07_1 0.1_13
Ireland Mytilus edulis 2016 G EtOH 1 417337 298547 0.2 0.009 0.003 0 0.01 91 7
Ruditapes philippinarum 2014 DG EtOH 2 279012_354851 221600_274155 55_61 0.11_0.15 0 0_0.01 0_0.3 0.3_0.4 38_44
G EtOH 1 25194 13342 2 0 0 0.02 0.01 68 29
Pecten maximus 2016 V EtOH 4 126068_572994 91340_417156 0.08_05 0 0.3_7 0_0.01 0.6_5 49_69 28_37
Italy Mytilus galloprovincialis 2018 G EtOH 2 20620_24086 3481_3820 59_70 0_0.08 0 0 0 0.6_1 28_40
Callista chione 2011 V FFPE 3 3992_26430 1606_11407 1_29 0_1 0 0_1.6 0_13 14_48 18_55
Donax trunculus 2018 V FFPE 2 8415_13030 864_4680 6_67 0_0.2 0 0 0 13_38 19_55
Patella caerulea 2018 V FFPE 1 15720 9858 2 0.3 0 0 0 19 78
Spain Mytilus galloprovincialis 2013 V EtOH 2 36506_190172 17380_142898 52_67 0_0.01 0 0_0.01 0 0.3_1 31_47
Cerastoderma edule 2013 V FFPE 1 24968 2670 28 0 0 0 0 14 57
2014 V EtOH 1 925235 768328 90 0 0 0.001 0 0.009 9
Mimachlamys varia 2012 V EtOH 2 83588_405889 56110_279185 0_17 0_0.05 5_8 0.003_0.06 0 13_16 60_78
Polititapes aureus 2010 V FFPE 1 2162 531 31 0 0 0 0 57 10
Norway Ostrea edulis 2017 DG EtOH 4 84350_268876 9646_41698 0.1_2 32_70 0 0 0_0.01 2_16 23_51
Chile Mytilus chilensis 2016 V FFPE 4 12087_64271 2631_28234 2_34 0_0.1 0_1.5 0_0.4 0_12 5_74 18_59
Argopecten purpuratus 2014 V FFPE 2 3306_12636 1083_1272 21_38 4_6 0_0.4 0_3 0 20_27 32_44

ELO: Endozoicomonas-like organisms, Anaplasma group. Myco: Mycoplasma group. Franc: Francisella group. Other: shows the minimum and maximum proportion of reads with similarity to other symbionts, grouped either with Chlamydia, Bacteroidetes, Spirochetes or sulfur-oxidizing endosymbiotic bacteria. Envirom: environmental, shows the minimum and maximum proportion of reads that were not identified as symbionts and were then classified as environmental. Unclass: unclassified, shows the minimum and maximum proportion of reads per sample that were not included within the top ten percent of reads in any one sample or which were not identified as the most abundant in any one sample and which were therefore not investigated. T: tissue analyzed as follows: “G” gill; “D” digestive gland; “M” mantle, and “V” various organs (for DNA extracted from FFPE). Fix: DNA extracted from ethanol fixed (EtOH) or from formalin-fixed and paraffin-embedded (FFPE) tissues. N°: number of samples sequenced per group. Details of the metadata associated with each sample can be found in Supplementary Table 1.