Table 3.

Dose response of Sargassum fusiforme on in vitro gas profile.

Parameters	Sargassum fusiforme					SEM	P value
	0%	1%	3%	5%	10%		T	L	Q
Total gas emission (mL g−1 DM)
3 h	153.97a	149.38c	154.70a	144.39d	151.50b	0.48	< 0.0001	0.0016	< 0.0001
6 h	163.02	168.25	163.82	160.97	160.86	3.88	0.6702	0.3348	0.8848
9 h	172.64	182.46	171.26	167.09	172.58	5.34	0.4009	0.4482	0.3684
12 h	174.27	177.60	182.14	186.73	178.45	5.53	0.5841	0.5775	0.1407
24 h	223.02a	195.65b	218.78a	209.08ab	203.57b	3.42	0.0003	0.0516	0.8033
48 h	232.09ab	238.22ab	240.44a	234.26ab	225.33b	3.89	0.1327	0.0679	0.1001
72 h	248.83	247.67	248.30	244.71	254.16	3.49	0.4681	0.2770	0.1930
Methane emission (mL g−1 DM)
3 h	1.70a	1.17b	1.59a	1.03b	0.92b	0.07	< 0.0001	< 0.0001	0.3310
6 h	1.89	2.22	1.86	1.90	1.98	0.31	0.9204	0.8983	0.8100
9 h	2.56	3.30	2.58	2.31	2.94	0.43	0.5499	0.9886	0.3912
12 h	3.30	3.47	4.20	4.61	3.88	0.55	0.4751	0.3842	0.1234
24 h	8.38a	4.86c	7.75ab	6.81ab	5.92bc	0.44	0.0003	0.0682	0.6578
48 h	9.96	11.16	12.89	10.77	10.86	1.35	0.6443	0.9397	0.3829
72 h	13.58	13.17	15.63	13.27	14.22	0.89	0.3401	0.6701	0.6243
Carbon dioxide emission (mL g−1 DM)
3 h	15.03a	11.55bc	13.69ab	9.67 cd	8.44d	0.55	< 0.0001	< 0.0001	0.2310
6 h	14.68	16.94	14.82	15.21	15.81	1.67	0.8692	0.9290	0.8413
9 h	18.96	21.86	18.98	17.24	20.82	2.16	0.6133	0.9393	0.3440
12 h	24.41	22.63	23.92	25.78	22.55	1.92	0.7426	0.7721	0.4211
24 h	38.88a	28.68c	35.79ab	33.11bc	29.37c	1.13	< 0.0001	0.0015	0.8011
48 h	43.07	45.14	50.22	44.21	48.21	4.08	0.7187	0.5167	0.7508
72 h	52.19ab	49.75b	60.25a	50.27b	52.21ab	2.48	0.0772	0.9288	0.3372

SEM, standard error of the mean, n = 3; DM, dry matter; T, treatment; L, linear; Q, quadratic.

^a,b,c,dMeans in a row followed by different superscript letters are significantly different (P < 0.05).