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. 2020 Nov 12;10:19738. doi: 10.1038/s41598-020-76778-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Particle properties of developed NPs. (A) Size distribution profiles, SEM images, and TEM images of PLGA/PHL NPs and DOX@PLGA/PHL NPs. Diameter-dependent differential intensity (%) profiles are shown as size distribution diagrams. (B) Incubation time-dependent particle size values of DOX@PLGA/PHL NPs in different media (DW, PBS (pH 7.4), and FBS (50%, v/v)). Each point indicates the mean ± SD (n = 3). Particle size distribution profiles, shown as diameter-dependent differential intensity values, of DOX@PLGA/PHL NPs in PBS (pH 7.4) and FBS (50%, v/v) mixture are shown. (C) Release profiles of PHL and DOX from PLGA/PHL NPs and DOX@PLGA/PHL NPs at pH 7.4 and 5.5. Each point indicates the mean ± SD (n = 3).