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. 2020 Nov 12;10:19636. doi: 10.1038/s41598-020-76416-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CCR2 and TF mRNA expression in different transfection agent treated cells. The TF and CCR2 mRNA expression were determined by Q-PCR. The experiments were performed in triplicate (mean ± SD). The mRNA levels from the different nanocarrier treated cells were normalized to the GAPDH mRNA level. The relative fold of the TF and CCR2 mRNA levels from the nanocarrier treated Raw 264.7 was normalized to the negative control from the normal cells. *P < 0.05, **P < 0.05, ***P < 0.05, ****P > 0.05.