Figure 3.

Sulforaphane-reduced phosphorylation of STAT3 leads to the downregulation of B7-H1. (A) Monocyte-derived dendritic cells (MoDCs) were pretreated with vehicle control, indicated concentrations of sulforaphane (SF) or respective pathway inhibitors for 30 min. Then cytokine cocktail (Cyt) or vehicle control was added into the cultures. Cells were collected 48 h later, labeled with B7-H1-PE, CD80-FITC, CD83-APC, or CD86-PE antibody and analyzed by flow cytometry and FlowJo software. Data are presented in Mean fluorescence intensity (MFI) ± SD, *P ≤ 0.05, n = 4-7. (B) MoDCs were treated with sulforaphane or vehicle control for 15 min, 30 min and 60 min, activated by cytokine cocktail, the proteins were harvested and phosphorylation of STAT3 was examined by western blot. The expression of β-actin served as a control for equal total protein. The band intensities (pixel intensities) were evaluated using ImageJ software after normalization to β-actin, n = 3, *P ≤ 0.05.