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. 2020 Nov 12;10:19657. doi: 10.1038/s41598-020-75013-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Few Ig transcripts were detected in PTECs by high-throughput single-cell sequencing. (a) The plot shows a two-dimensional representation (tSNE t-distributed stochastic neighbor embedding) of global relationships among 6138 single cells. A total of 11 clusters were identified, among which PTECs accounted for 6.1% (387/6138). Loupe Browser (version 4.0.0) was used for clustering. (b) Expression of PTEC marker genes (CUBN and LRP2) colored on the basis of normalized expression levels (gray, low; red, high). (c) Only five Ig transcripts were detected in five PTECs. The horizontal axis represents 387 single PTECs and the vertical axis represents five Ig genes (blue, no expression; red, expression).