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. 2020 Nov 12;10:19657. doi: 10.1038/s41598-020-75013-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transcripts of the Igγ chain were detected in sorted PTECs by FACS. (a) Schematic overview of the single-cell sequencing workflow in PTECs. (b) PTECs were sorted by FACS using antibodies against CD10-PE and CD13-APC. FACS analysis revealed that 4.3% of cells were double-positive. (c) PCR analysis of three PTEC marker genes: CD10, CD13 and LRP2; cDNA from the kidney cortex was used as a positive control. (d) PCR analysis of Igγ, RAG1, RAG2 and the B cell marker gene CD19. Peripheral blood mononuclear cells (PBMCs) were used as the positive control. Water instead of cDNA was used as a negative control. The Numbers (1 to 6) refer to six randomly selected PTEC cells for presentation. The full-length gels were presented in Supplementary Fig. 1. (e) IgG positive detection rate in PTECs from 2 patients was 91.8%, 74.2%, respectively.