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. 2020 Nov 12;10:19657. doi: 10.1038/s41598-020-75013-9

Figure 3.

Figure 3

PTEC-derived IgG presents some basic characteristics similar to B-IgG. (a–c) Percentages of single cells expressing one, two, or three VHDJH segments. (a) In 45 single PTECs from patient 1 expressing IgG, two PTECs expressed two VHDJH patterns, and one expressed three VHDJH patterns. (b) In 46 single PTECs from patient 2 expressing IgG, eight PTECs expressed two VHDJH patterns, and one expressed three VHDJH patterns. (c) In 3084 single B cells expressing productive IgG in Shi’s article, 86 single B cells expressed two IgG VHDJH patterns, and none expressed three VHDJH patterns. (d–f) PTEC-derived IgG contained classic somatic hypermutation. Distribution of somatic hypermutations (d) and hotspot mutations (e) in Igγ variable region genes. Somatic hypermutations (f) were concentrated in the CDR but not in the FRs. A paired t-test was used to compare the mutation frequency between the CDR and FR. ****, p < 0.0001.