Figure 2.

Streptococcus parasanguinis alters the structure of mixed biofilms. Confocal scanning laser microscopy images of wild-type S. mutans, C. albicans, and S. parasanguinis single and mixed biofilms at 60X magnification. S. mutans was labeled with green fluorescent protein (GFP), C. albicans was stained with calcofluor white, and S. parasanguinis was labeled with mCherry. All biofilms were grown in tryptic soy broth containing 0.5% yeast extract (TSBYE) and 1% sucrose for 16 h at 37 °C with 5% CO₂. Scale bar: 25 µm. Images were acquired using the Nis Elements 5.0 Imaging Software (https://www.microscope.healthcare.nikon.com/products/software/nis-elements).