Figure 6.

Streptococcus parasanguinis blocks synergy between GTFs and C. albicans. (A) Crystal violet assay of C. albicans biofilms with supernatant from wild-type or the gtfB mutant strain of S. mutans. (B) Crystal violet assay of C. albicans and C. albicans–S. parasanguinis biofilm with purified GTFs from overnight S. mutans cultures. *P = 0.0246 ****P < 0.0001; (C) Confocal laser scanning microscopy images of C. albicans biofilm stained with calcofluor white with GTFs or GTFs and S. parasanguinis. Scale bar: 50 µm. All cultures were grown in tryptic soy broth containing 0.5% yeast extract (TSBYE) and 1% sucrose for 16 h at 37 °C with 5% CO₂.