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Streptococcus parasanguinis blocks glucan formation by GTFs. Confocal laser scanning microscopy of purified GTFs from S. mutans culture and cascade Blue-labeled dextran in (A) TSBYE, TSBYE + 1% Sucrose, or TSBYE + 1% sucrose with 100 µL of S. parasanguinis supernatant (SN). (B) Fluorescence intensity of each biofilm. ****P < 0.0001 Scale bar: 50 µm. All cultures were grown for 16 h at 37 °C with 5% CO2.
