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. 2020 Nov 12;11(11):976. doi: 10.1038/s41419-020-03185-3

Fig. 1. p53 positively regulates the expression of DEPTOR under unstressed conditions.

Fig. 1

a, b Silencing of p53 decreases the expression of DEPTOR at both protein a and mRNA levels b. Multiple cancer cell lines harboring wild-type p53 were transfected with siRNA targeting p53 or control scrambled siRNA for 48 h and then subjected to immunoblotting (IB) with the indicated antibodies (Abs) a or quantitative reverse transcription (qRT-PCR) analysis b (mean ± S.E.M, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). c–f p53 knockout reduces the expression of DEPTOR at both protein c, e and mRNA levels d, f. HCT116 and U2OS cells, with or without p53 deletion, were harvested for IB with the indicated Abs c, e or qRT-PCR analysis d, f (mean ± S.E.M, n = 3; **p < 0.01, ***p < 0.001). g, h DEPTOR expression is decreased at both the protein g and mRNA levels h in multiple tissues from Trp53−/− mice. The indicated tissues from littermates were homogenized and subjected to IB with the indicated Abs g or qRT-PCR analysis h (mean ± S.E.M, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). The band density was quantified and expressed as fold change compared with the corresponding control by setting the control value as 1 a, g.